1. What is a Sequencing Coverage Calculator?

Definition: This calculator estimates the sequencing coverage based on read length, number of reads, and genome size.

Purpose: It helps researchers determine the depth of sequencing needed for their genomic studies.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( C \) — Coverage (dimensionless)
• \( L \) — Read length (base pairs)
• \( N \) — Number of reads (dimensionless)
• \( G \) — Genome size (base pairs)

Explanation: The product of read length and number of reads gives total bases sequenced, which when divided by genome size gives coverage.

3. Importance of Coverage Calculation

Details: Proper coverage estimation ensures sufficient data quality for variant detection and genome assembly.

4. Using the Calculator

Tips: Enter the read length in base pairs, number of reads, and genome size in base pairs. All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is considered good coverage?
A: For human genomes, 30x is standard for whole genome sequencing, while 100x may be needed for cancer studies.

Q2: How does read length affect coverage?
A: Longer reads provide better coverage with fewer reads but may have higher error rates.

Q3: What if my genome size is unknown?
A: Use estimated size from closely related species or consult genomic databases.

Q4: Does this account for paired-end reads?
A: No, for paired-end sequencing, multiply number of reads by 2 before calculation.

Q5: How does coverage relate to sequencing depth?
A: Coverage is synonymous with sequencing depth - both describe how many times each base is sequenced on average.